Background: Sleep disturbances are common in people living with dementia and negatively affect them and their family carers. Non-pharmacological interventions like DREAMS:START (Dementia Related Manual for Sleep: Strategies for Relatives) offer potential benefits but are not yet implemented widely in NHS practice. Aims: To explore the barriers and facilitators to implementing DREAMS:START in UK NHS […]

Recognition of the branch sequence (BS) by U2 snRNP is a pivotal step in pre-mRNA splicing and spliceosome assembly. Structural studies suggest that BS recognition occurs through a toehold-mediated strand-invasion mechanism, in which U2 snRNA progressively base-pairs with the intron to form the branch helix. However, given the limited complementarity between U2 snRNA and the […]

The brown snake, Storeria dekayi, is distributed across southeastern Canada, the eastern United States, and eastern Mexico, with isolated records in Central America. Despite its broad range, S. dekayi is currently recognized as a single species. Historically, eight subspecies were described based primarily on head coloration patterns, but subsequent genomic analyses of populations from the […]

Fluorescent in situ sequencing involves imaging-based sequencing by synthesis in intact cells or tissues to reveal target nucleotide sequences inside each cell. Often, the target sequences are barcodes that indicate a perturbation (e.g. CRISPR guide or genetic variant) delivered to the cell. However, processing in situ sequencing data presents a considerable challenge, requiring stitching and […]

Bacteriophage anti-CRISPR (Acr) proteins have the potential to reduce off-target effects of genome editing by inactivating the CRISPR-Cas bacterial defense. The current challenge lays in their functional annotation, as Acr proteins have high structural diversity and low sequence similarity, thus rendering common homology-based methods unfit. Recent solutions use deep learning models such as graph convolutional […]

Interaural time and level differences are crucial in sound localization, yet their contributions to sound source segregation and spatial selection remain underspecified. Here, participants completed a spatial auditory selective attention task while we measured hemodynamic activity in the prefrontal cortex and superior temporal gyrus using functional near-infrared spectroscopy. Participants listened to a target sound stream […]

Intimate Partner Violence (IPV) remains a significant global public health concern, with youths disproportionately affected compared to the general population of women aged 15-49 years. In Tanzania, nearly one in two (47.8%) female youth reported having experienced IPV in 2015. Despite national interventions, help-seeking behavior among this group remains low. The study was an analytical […]

Symbiotic relationships transform diverse aspects of both symbiont and host biology. The most visible changes include a massive reduction of the endosymbiont genome and the development of novel host organs, cells, and compartments specialized for harboring the symbionts. However, many insect symbiosis studies have previously focused either on Proteobacteria or only on a particular symbiosis […]

Importance: Traditional LDL-C testing barriers – mandatory 9-12 hour fasting and in-person visits – disproportionately limit access for rural populations (60% of US counties lack cardiology services), shift workers, patients with diabetes, and caregivers. These barriers persist despite 2016 European guidelines endorsing non-fasting lipid panels and explosive post-COVID telehealth growth. Objective: To determine whether machine […]

The liver exhibits striking sexual dimorphism in gene expression that impacts drug and lipid metabolism and disease susceptibility, with males showing substantially higher predisposition to metabolic dysfunction-associated steatotic liver disease (MASLD) and its complications including hepatocellular carcinoma. These sex differences are primarily controlled by sexually dimorphic pituitary growth hormone (GH) secretion patterns; however, the underlying […]