The widespread use of antibiotics in broiler production has contributed to antimicrobial resistance, necessitating the development of sustainable alternatives. Synbiotics, combining probiotics and prebiotics in a synergistic formulation, have emerged as promising candidates to replace antibiotics. This study evaluated the efficacy of dietary synbiotics as alternatives to early-life antibiotic supplementation in broiler chickens, examining their […]
Evolutionary Adaptation of Prephenate Dehydrogenases: A regulatory ACT domain acquisition in ecological niche specialization
Bacteria prephenate dehydrogenase (PDH) participates in the metabolic pathway for tyrosine biosynthesis. PDHs within the Bacilaceae phylum contain an ACT domain which enables them to be allosterically regulated by tyrosine. The mechanism via which the ACT domain introduces allostery onto PDH enzymes remains elusive. Furthermore, the evolutionary and biological advantages of ACT domain mediated regulation […]
Echo-planar imaging-based time-of-flight imaging using a modified interleaved flyback trajectory
1.1 Purpose A modified interleaved flyback (miFB) approach is introduced, designed to mitigate flow artifacts caused by alternating readout polarities in Echo Planar Imaging (EPI), while preserving acquisition efficiency. 1.2 Methods We propose reconstructing odd and even echoes of 3D-EPI separately. To this end, the respective missing lines are acquired in interleaved shots with inverted […]
Robust Colonization of Nasal Microbiota Across the Respiratory Tract and the Gut Modulates Innate Immune Response in a Germ-Free Pig Model
The use of antibiotics in swine production has contributed to improved health and growth rates but also led to significant challenges, including the emergence of antibiotic-resistant bacteria and disruptions in the microbiome. This study employs a germ-free pig model to investigate the transplantation of the nasal microbiome into the respiratory tract, exploring its potential to […]
Womens autonomy in consent and debriefing for caesarean section: a thematic analysis of healthcare providers views and womens lived experiences and expectations in 20 hospitals across West Cameroon
Womens autonomy in providing informed consent for caesarean section (CS) and receiving post-operative debriefing is essential for a high-quality, women-centered experience of care. Yet, in many low-income settings, while the clinical quality of CS is known to be suboptimal, evidence on its woman-centredness remains limited. Our study examined womens autonomy around CS in 20 hospitals […]
Rationally designed minimized TbpB confers broad protection against meningococcal infection
Transferrin binding protein B (TbpB), an iron acquisition protein, has long been recognized as a promising vaccine candidate targeting the pathogenic Neisseria species, including Neisseria meningitidis, the cause of meningococcal disease, and Neisseria gonorrhoeae, the cause of gonorrhea. A challenge to the development of this protein as a vaccine immunogen is the extent of antigenic […]
Ticking Differently: Elucidating Sexual Dimorphism in Human Aging through Metabolomics, Proteomics and Genomics
That males and females age differently has been overlooked while developing aging clocks. Here, we developed a sex-specific metabolic aging clock in 390,941 individuals from the UK Biobank and integrated it with genetic, proteomic and epidemiological data to identify mechanisms accelerating/decelerating metabolic aging in males and females. Our findings reveal dysregulation of cholesterol metabolism, immune […]
Structural basis of HSV-1 DNA replication origin-binding protein UL9
The herpesvirus DNA replication machinery comprises a battery of viral enzymes that orchestrate viral genome synthesis. In HSV-1, the machinery consists of seven essential components, including the replication origin-binding protein UL9, ssDNA-binding protein ICP8, the heterodimeric DNA polymerase complex UL30/UL42, and the heterotrimeric helicase-primase complex UL5/UL8/UL52. UL9, an SF2 helicase family member, functions as a […]
From Health Needs to the Use of Health Services: A Scoping Review Protocol on Access to Medicines
This study aims to analyze the relationship between health needs (felt and expressed) and access to medicines. It is a scoping review based on the Joanna Briggs Institute (2024) guidelines, structured according to the PCC strategy (Population, Concept, and Context). Searches will be conducted in PubMed, SciELO, LILACS, Scopus, and Embase for articles published between […]
Exploring the Restoration of Memory Post-NeuronalRegeneration from Induced ALS-FTD in Dugesia tigrina
Amyotrophic lateral sclerosis-frontotemporal dementia (ALS-FTD) is a neurodegenerative disease characterized by progressive neuronal loss and motor decline. Recovery remains beyond therapeutic reach, and even complete neurogenesis does not ensure preservation of memory capacity (defined as the strength of synaptic connections). Freshwater planarians, capable of whole-body regeneration and associative learning, provide a tractable model to study […]