We investigated whether T cell responses and antigen-presenting cell (APC) requirements in gut-draining lymph nodes differ by antigen source, diet versus epithelium. Using mice fed ovalbumin (OVA) or expressing secreted (s), cytosolic (c), or transmembrane ™ epithelial OVA, we compared OVA-specific T cell fates. At baseline and after reovirus infection, T cell responses were comparable […]
Assessing Compliance with Smoke-Free Laws in Purbakhola Rural Municipality, Nepal: A Cross-Sectional Observational Study
Introduction Despite Nepal’s comprehensive tobacco control legislation, evidence on its implementation at the local level, particularly in rural areas, remains scarce. This study assessed compliance with smoke-free laws in public places within Purbakhola Rural Municipality, Palpa, Nepal. Methods A cross-sectional observational survey was conducted from May, 2025, across all six wards of the municipality. A […]
GONNECT: A Gene Ontology-guided Neural Network for Explainable Cancer Typing
Deep Neural Networks (DNNs) are renowned for their high accuracy and versatility, which has led to their application in many fields of research, including biology. However, this accuracy often comes at the expense of interpretability, making it challenging to reason about the inner workings of most DNNs. Particularly in biological research, understanding the mechanisms behind […]
Evaluation of HEART score, GRACE 2.0 score, and TIMI score for Risk Stratification in Acute Coronary Syndrome: A Single-Centre Pilot Study in a Sri Lankan Population
Objectives HEART score is widely used for risk stratification in patients with chest pain to rule out acute coronary syndrome (ACS). Although limited, it has been validated in several countries throughout the world. TIMI and GRACE 2.0 scores are recommended for prognostication in patients with ACS. This study aimed to explore the effectiveness of HEART […]
NF-kB-Dependent Transcriptional Regulation of Piezo1 Mediates Bacterial Clearance on Stiffened Lung
Respiratory pathogens, such as Pseudomonas aeruginosa damage the alveolar-capillary barrier leading to lung injury and stiffness. Lung stiffness is a key macrophage signal for bacterial clearance, but it remains unknown how stiffness-sensing mechanosensitive ion channels in macrophages are regulated during pneumonia. Macrophage Piezo1 is critical to bacterial clearance in experimental pneumonia in vivo; however, identification […]
Evaluation of Cardiac Complications due to Mannitol Application in Elevated Intracranial Pressure patients: A Systematic Review and Meta-analysis
Background: Mannitol is a widely used hyperosmotic agent for intracranial pressure (ICP) control, yet its cardiovascular safety profile remains uncertain. This systematic review and meta-analysis aimed to evaluate cardiac complications and mortality associated with mannitol administration in patients with elevated ICP. Methods: Following PRISMA 2020 guidelines, five databases (PubMed, Scopus, Web of Science, Embase, and […]
Detection of five viruses commonly implicated with Bovine Respiratory Disease using loop-mediated isothermal amplification
Herein, we present novel quantitative loop-mediated isothermal amplification (qLAMP) and reverse-transcription qLAMP (RT-qLAMP) assays for the detection of five viruses commonly implicated with the onset and progression of bovine respiratory disease (BRD): Bovine Alphaherpesvirus Type 1 (BHV-1), Bovine Adenovirus Type 3 (BAV-3), Bovine Respiratory Syncytial Virus (BRSV), Bovine Viral Diarrhea Virus Type 1 (BVDV-1), and […]
Social media language patterns reveal early signs of impending stroke: An observational study
Background: Stroke remains a major global health concern, contributing substantially to mortality and long-term disability. Current clinical tools lack effective mechanisms for early detection. Here, we investigate whether linguistic and behavioral patterns in online social media can serve as early indicators of impending stroke. Methods: We analyzed posts from 1,683 Reddit users who reported experiencing […]
Ketamine and Psilocybin Differentially Impact Sensory Learning During the Mismatch Negativity
Ketamine and psilocybin show potential as therapies for various mental illnesses, including major depressive disorder. However, further investigation into their neural mechanisms is required to understand their effects on the brain. By combining computational modelling with electroencephalography (EEG), we examine the effects of ketamine and psilocybin on hierarchical sensory pwPE learning in the context of […]
Palmar cooling mitigates exercise-induced immune suppression after high-intensity training: A randomized trial
Objectives We investigated whether palmar cooling alters inflammatory responses following a single session of high-intensity eccentric exercise. We hypothesized that palmar cooling during rest intervals would attenuate maladaptive inflammatory responses while preserving beneficial immune adaptations necessary for muscle repair and recovery. Methods In this randomized interventional study, 20 healthy adults were matched by sex and […]