When recalling past episodes, different features of an experience, such as conceptual meaning and perceptual detail, are reconstructed and reinstated across distributed cortical regions. However, current models of human memory remain unclear how these feature-specific reinstatements unfold over time, and whether they interact hierarchically during memory retrieval. Using magnetoencephalography (MEG) and time-resolved encoding-retrieval cross-phase classification […]

Montanine, an Amaryllidaceae alkaloid, has demonstrated superior antiviral activity against dengue virus (DENV), SARS-CoV-2, vesicular stomatitis Indiana virus (VSV-G), and vesicular stomatitis virus pseudotyped with Ebola virus glycoprotein (VSV-ZGP), outperforming other antiviral alkaloids tested, including lycorine, narciclasine, tetracetylnarciclasine, and pancracine. We further showed that montanine inhibits translation in mammalian cells, as evidenced by a puromycin […]

Background: Air pollution caused by fine particles has been recognized as a significant environmental risk factor. Over the past two decades, there has been a substantial increase in evidence on the impact of air pollutants on mortality and morbidity in vulnerable groups, such as children under 5 years of age, people over 60 years of […]

Nanoflow electrospray ionization is commonly used for proteomics due to its high sensitivity. Signal intensity, however, is dependent on optimal emitter positioning relative to the mass spectrometer inlet. Here, we characterize the effect of varied emitter positions on peptide signal intensity in all three dimensions using emitters and flows consistent with standard proteomic analyses. We […]

The effective titer and quality of biopharmaceutical products can be enhanced by genome engineering of producer cell lines; however, licensing constraints often limit nuclease utility. Here, we validate OpenCRISPR-1 in CHO cells by achieving [≥]70% INDEL efficiency across multiple genes. We demonstrate biallelic Fut8 knockout in monoclonal cell lines with 31% efficiency and quadruplex knockout […]

Mass spectrometry (MS)-based metabolomics has emerged as a powerful tool to address multifaceted biological questions. Commercial solutions like the ones developed at biocrates allow reliable and quantitative targeted metabolic profiling, including the conversion of the raw MS spectra into absolute concentrations of metabolites. These results can be exported for further analysis under several formats with […]

Introduction Urinary tract infections are common to pregnant and nonpregnant women estimated to 150 million new cases annually. The incidence increases with pregnancy due changes that take place. Causative microbes are E.coli, Klebsiella pneumoniae and Staphylococci species. The disease presents symptomatically or asymptomatically, early investigation, detection and treatment to pregnant mothers are crucial to avoid […]

Chymotrypsin is widely used in shotgun proteomics owing to its orthogonal cleavage specificity relative to trypsin, which enhances sequence coverage of hydrophobic protein regions. However, commercial preparations often display variable cleavage specificity, trypsin contamination, and elevated missed cleavage rates, which can collectively reduce proteome coverage and data reproducibility. To address these limitations, we present a […]

UreE is a nickel chaperone required for the safe and efficient delivery of nickel to the active site of the metalloenzyme, urease; a key virulence factor of the urinary tract pathogen, Proteus mirabilis. We investigated the structural features of P. mirabilis UreE using protein X-ray crystallography and its nickel-binding capacity by inductively coupled plasma-mass spectrometry. […]

Background and aims The utility of cardiovascular and inflammatory biomarkers to detect coronary obstruction and predict survival benefit of revascularisation in chronic coronary syndrome (CCS) remains unclear. Methods Patients undergoing coronary angiography for suspected CCS were studied. High-sensitivity cardiac troponin T (hsTnT), N-terminal pro-B-type natriuretic peptide (NT-proBNP), high-sensitivity C-reactive protein (hsCRP), interleukin-6 (IL-6), and copeptin […]