Background: Pharmacist-led anticoagulation management services (AMS) for direct oral anticoagulants (DOACs) reduce prescribing errors and enhance adherence, but have not demonstrated lower rates of stroke or bleeding compared to usual care, and their cost-effectiveness is unknown. We evaluated four anticoagulant strategies for patients with atrial fibrillation initiating therapy: warfarin AMS, DOAC usual care, DOAC population […]
Alveolar-Basal Intermediates Drive Pulmonary Fibrosis via Coordination of a Pro-Fibrotic Signaling Niche in Silicosis
Pulmonary fibrosis is a progressive, terminal disease with high mortality. Existing therapeutics are capable of slowing disease progression but are unable to reverse fibrotic lung remodeling, accentuating the importance of studying the mechanisms that underlie lung resilience and repair during lung fibrosis. Recent literature has suggested that alveolar type 2 (AT2) progenitors undergo transition to […]
Ac-DOTA-TATE in SSTR2-positive tumor models
Somatostatin receptor 2 (SSTR2) is highly expressed in neuroendocrine tumors including small cell lung cancer (SCLC) and represents a validated target for peptide receptor radionuclide therapy. The SSTR2 agonist [177Lu]Lu-DOTA-TATE is clinically approved, however, treatment resistance and relapse occur. The SSTR2 antagonist SSO110 (DOTA-JR11, OPS201) demonstrates higher tumor uptake and longer retention than DOTA-TATE both […]
Modelling mass asymptomatic testing strategies for early containment of infectious disease outbreaks in prisons
Objective: Investigate a strategy of mass asymptomatic testing and isolation ("pulse testing") aimed at early containment of outbreaks in prisons in comparison to or combination with a symptom-based isolation strategy. Methods: Simulations using an individual-based time-since-infection model were run under different pathogen and intervention strategy scenarios. Measured outcomes were the proportion of outbreaks contained and […]
Empagliflozin preserves cardiac function and modulates metabolism in a mouse model of Duchenne muscular dystrophy
Duchenne muscular dystrophy (DMD) is a fatal genetic disorder characterized by skeletal muscle degeneration and cardiomyopathy without a cure. This study examined the therapeutic potential of sodium-glucose cotransporter 2 (SGLT2) inhibitor empagliflozin (EMPA) on cardiac function in the dystrophin-deficient mdx mouse model of DMD. Male mice received EMPA (~25 mg/kg/day) chow or control chow, and […]
Combining Anion Exchange and Size Exclusion Chromatography for Extracellular Vesicle Enrichment from Small Volumes of Human and Mouse Plasma for Quantitative Proteomics
Extracellular vesicles (EVs) are promising biomarkers, yet their proteomic analysis from plasma is hampered by low abundance and co-purification of contaminants (e.g., lipoproteins, platelets) and technical variability, particularly in small-volume animal models. We developed and validated a modular protocol integrating Size Exclusion Chromatography (SEC) with Strong Anion Exchange (SEC-SAX) specifically tailored for quantitative LC-MS proteomics […]
Precise kinematic and muscle recording in freely behaving flies enabled by closed-loop tracking and annotation-free pose estimation
Understanding the neuromuscular basis for behavior requires measuring both kinematic and physiological data at high resolution in unconstrained conditions: a technically challenging goal. Here we present an integrated experimental-computational pipeline for measuring and quantifying body part kinematics and muscle activity in freely behaving Drosophila melanogaster. We first present Spotlight, a closed-loop videography system that performs […]
Pre-trained Vision Transformers for Seizure Prediction: A Reproducible Baseline with Event-Based Evaluation and Statistical Validation
Background: Scalp electroencephalography (EEG) based seizure prediction plays a critical role in improving the quality of life for patients with drug-resistant epilepsy, offering the potential for real-time warnings and timely interventions. Despite its clinical significance and decades of research, the field still lacks an open benchmark with reproducible baselines and deployment-oriented event-level evaluation. Most prior […]
Automated culture and monitoring of a high-throughput human heart-on-a-chip
Organ chips offer a disruptive innovation to study human diseases with tissue-specific resolution within a predictable and tunable in vitro environment. However, these platform technologies have for the most part failed to translate to broad use in the private sector due to a lack of high-throughput, user-friendly platforms. Here we present an automated high-throughput organ […]
Feasibility and safety of long-term remote-only monitoring in a large cohort of pacemaker patients
Background Remote monitoring (RM) enables convenient follow-up of patients with cardiac implantable electronic devices. In remote-only monitoring (RM-only) in-person visits are only scheduled if needed based on RM findings. This study evaluated the feasibility and safety of long-term RM-only of pacemaker (PM) patients. Methods All patients with Biotronik PM were included in the analysis. Data […]
Evaluating the efficacy and cost-effectiveness of a digital, app-based intervention for depression (VMood) in community-based settings in Vietnam: A stepped-wedge cluster randomized controlled trial
Depression contributes substantially to the global burden of disease, with large treatment gaps, particularly in low- and middle-income countries (LMICs) like Vietnam. Digital mental health interventions (DMHIs) offer a promising solution, yet evidence for the efficacy and cost-effectiveness of digitally delivered adaptations of evidence-based interventions in LMICs remains limited. This trial evaluated the efficacy and […]
Nutraceutical and functional potential of the Apis mellifera L. royal pupae proteome
The objective of this research was to characterize the proteome of the Apis mellifera royal pupae to evaluate its potential as a nutraceutical and functional food. Six pupal instars (E1 to E6) were analyzed using liquid chromatography, mass spectrometry, and bioinformatics techniques to determine their properties and biological functions. The results showed 15 proteins across […]