Background: Antimicrobial resistance (AMR) is a growing global health concern driven largely by inappropriate antimicrobial use. Antimicrobial stewardship programs (ASPs), guided by the Centers for Disease Control and Prevention (CDC) core elements, are essential for optimizing antimicrobial use. However, adherence to these practices and the barriers faced by healthcare workers remain inadequately explored, particularly in […]
A Comparative Study in Surgical AI: Datasets, Foundation Models, and Barriers to Med-AGI
Recent Artificial Intelligence (AI) models have matched or exceeded human experts in several benchmarks of biomedical task performance, but have lagged behind on surgical image-analysis benchmarks. Since surgery requires integrating disparate tasks — including multimodal data integration, human interaction, and physical effects — generally-capable AI models could be particularly attractive as a collaborative tool if […]
Incidence, Clinical Features, and Outcomes of Acute Kidney Injury in Adults and Children Admitted with Dengue Infection in Jamaica
Background: The global burden of dengue infection has rising, yet limited data exists on its impact in the Caribbean. We describe the incidence and associates of acute kidney injury in adults and children with dengue at a teaching hospital in Jamaica. Methods: A single-centre retrospective cohort study of admissions with laboratory confirmed dengue infection at […]
Population-Level Distribution of PRISQ Scores among adults accessing primary care services in the state of Qatar
Background: Prediabetes is a critical intermediate stage in the development of type 2 diabetes mellitus and is increasingly prevalent in the Eastern Mediterranean Region. In Qatar, high levels of metabolic and lifestyle-related risk factors underscore the need for scalable, non-invasive risk stratification tools within primary care. The Prediabetes Risk Score in Qatar (PRISQ) was developed […]
CLIAMDK: A Modular Smartphone Platform Matching Plate Reader Performance for Chemiluminescent Immunoassay Development
The development of chemiluminescent immunoassays (CLIAs) is a complex and iterative process that relies on costly laboratory infrastructure, limiting its accessibility and application across healthcare settings and disease areas. Here, we detail the CLIA Mobile Development Kit (CLIAMDK) a modular, mobile, and inexpensive platform to assess image sensors, smartphones and data processing workflows for CLIA […]
CLIAMDK: A Modular Smartphone Platform Matching Plate Reader Performance for Chemiluminescent Immunoassay Development
The development of chemiluminescent immunoassays (CLIAs) is a complex and iterative process that relies on costly laboratory infrastructure, limiting its accessibility and application across healthcare settings and disease areas. Here, we detail the CLIA Mobile Development Kit (CLIAMDK) a modular, mobile, and inexpensive platform to assess image sensors, smartphones and data processing workflows for CLIA […]
Population-Level Distribution of PRISQ Scores among adults accessing primary care services in the state of Qatar
Background: Prediabetes is a critical intermediate stage in the development of type 2 diabetes mellitus and is increasingly prevalent in the Eastern Mediterranean Region. In Qatar, high levels of metabolic and lifestyle-related risk factors underscore the need for scalable, non-invasive risk stratification tools within primary care. The Prediabetes Risk Score in Qatar (PRISQ) was developed […]
Incidence, Clinical Features, and Outcomes of Acute Kidney Injury in Adults and Children Admitted with Dengue Infection in Jamaica
Background: The global burden of dengue infection has rising, yet limited data exists on its impact in the Caribbean. We describe the incidence and associates of acute kidney injury in adults and children with dengue at a teaching hospital in Jamaica. Methods: A single-centre retrospective cohort study of admissions with laboratory confirmed dengue infection at […]
Chromatoid body integrates piRNA, SMG6 and m⁶A pathways to control mRNAs in the male germline
Spermatogenesis requires tightly controlled transcriptome regulation, supported by the PIWI-interacting RNA (piRNA) and nonsense-mediated decay (NMD) pathways, both concentrated in the chromatoid body (CB) of haploid spermatids. We previously showed that the NMD endonuclease SMG6 interacts with the piRNA-binding protein PIWIL1, and that loss of either factor results in overlapping mRNA dysregulation, suggesting functional cooperation. […]
Identification and functional investigation of Octopus vulgaris TRPV channels as potential nociceptors in cephalopods
Nociception is an essential response for organisms to avoid potential harm and promote survival. Its molecular determinants are largely conserved across Eumetazoa. TRPV receptors are polymodal ion channels exhibiting selective peripheral expression and functional coupling that underpins nociception and pain modulation in complex organisms. However, the execution of protective behaviours triggered by TRPVs is also […]
Inhibition of V-ATPase function drives apoptosis via GCN1/GCN2 kinase signaling
Natural products are a rich source of bioactive molecules that have served both as templates for drug discovery and as tools to uncover fundamental biological processes. While characterizing the pro-apoptotic activity of the cyanobacterial metabolite Nostatin A, we identified vacuolar-type H+-ATPase (V-ATPase) as its molecular target and uncovered an unexpected signalling response preceding cell death […]
STING causes replication stress and nascent DNA degradation via SAMHD1
STING is a key innate immune adaptor, classically activated by cytosolic DNA via cGAS-cGAMP to induce type I interferon signaling. While its cytoplasmic role is well-defined, recent studies reveal that STING participates in non-canonical signaling pathways and localizes at the nuclear envelope and chromatin, where its functions remain poorly understood. In Hutchinson Gilford Progeria Syndrome […]