Medical clinical minds meet artificial intelligence: Italian physicians’ knowledge, attitudes, and concordance between Italian physicians and AI-generated diagnoses. A national cross-sectional study

BackgroundArtificial Intelligence has increasingly been integrated into clinical practice, yet its adoption and perception among medical professionals remain poorly understood, particularly in the Italian healthcare system. To investigate Italian physicians’ knowledge, attitudes, and clinical concordance with AI-generated diagnostic recommendations, using a validated questionnaire and a clinical scenario processed by ChatGPT.MethodsA national, cross-sectional web-based survey was […]

User experience design methodologies for developing a tele-round platform in public intensive care units in northern and northeastern Brazil

IntroductionDesigning digital health solutions for critical environments like intensive care units (ICUs) is challenging, especially in resource-constrained settings. The integration of user experience (UX) design methods into digital health development may improve alignment with clinical workflows, reduce barriers to adoption, and enhance perceived usefulness.ObjectiveTo apply user experience design methodologies to develop the interface of a […]

Real-world outcomes from 2,905 episodes of hospital at home care: a propensity-matched cohort study

BackgroundHospital at home (HAH) services within the UK have expanded rapidly over the last 5 years, but there is comparatively little evidence demonstrating their clinical effectiveness. In this study, we evaluated the clinical outcomes, safety, and cost-effectiveness of a comprehensive HAH service in England.MethodsWe conducted a retrospective cohort study of patients admitted to our HAH […]

Preparing real-world data through common data model harmonization of cancer patient records in the COMNet platform at the Modena Oncology Center

ObjectivesThe transition from paper medical records to electronic health records (EHRs) has enabled the extraction of substantial real-world data, which can support future real-world evidence generation. This study aimed to convert heterogeneous oncology data from local EHR systems—collectively referred to as COMNet—into a standardized data model. In particular, the Observational Medical Outcomes Partnership Common Data […]

A randomized, unblinded, controlled clinical study to assess the mobile digital health application INKA in the management of therapy refractory overactive bladder and mixed incontinence

BackgroundThis exploratory, two-arm, randomized, unblinded, controlled, multicentre study assessed the health benefits of the INKA app, a MDR class I CE-marked digital therapy companion for patients with overactive bladder (OAB) and mixed incontinence (MI). INKA offers self-guided educational, behavioural, and motivational content, along with physiotherapy modules and supports daily self-management, in accordance with current clinical […]

Creating customized chatbots with ChatGPT to promote physical activity: a mini review

Artificial intelligence (AI) chatbots powered by large language models (LLMs) such as ChatGPT offer a promising approach for delivering scalable, personalized physical activity interventions. Despite growing interest in applying these tools to health behaviour change, concerns remain regarding accuracy, safety, hallucinations, privacy, and theoretical grounding. This mini-review summarizes current methods for creating customized ChatGPT-based chatbots […]

Structural features of E. coli Stx bacteriophage phi24B revealed with cryo-electron microscopy

Shiga toxin-converting bacteriophages play a critical role in the emergence and virulence of pathogenic Escherichia coli strains. Despite their significance, detailed structural information on these phages remains scarce. Here we present a high-resolution cryo-electron microscopy and proteomic analysis of the phi24B bacteriophage, revealing an icosahedral capsid with T=9 symmetry, decorated by a processed esterase protein […]

Targeted sequencing of mutations via RNA-templated gap filling of oligonucleotides for single-cell RNA-seq

We describe a method for targeted sequencing of expressed mutations in single cells. The approach exploits the reverse transcriptase and nick translation activities of the Bst DNA polymerase (Full Length) from Bacillus stearothermophilus (BstFL) to perform an RNA-templated gap filling and ligation between flanking probes. Integrated with probe-based single cell RNAseq workflows, it enables simultaneous […]

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