Background: Improvements in health technology offer opportunities for remote disease screening, diagnosis and monitoring. The Withings Sleep Analyzer (WSA), an under mattress ballistocardiograph sensor able to detect body movement, breathing, and cardiac ejection is a promising technology for the non-invasive detection and monitoring of neurodegenerative diseases. InSleep46 aims to evaluate whether the WSA is able […]
Comparative Efficacy and Safety of Calcitonin Gene-Related Peptide Monoclonal Antibodies Versus Oral Gepants for Episodic Migraine Prevention: A Bayesian Network Meta-Analysis of Randomized Controlled Trials
Background. Calcitonin gene-related peptide (CGRP)-targeted therapies, including injectable monoclonal antibodies (mAbs: erenumab, fremanezumab, galcanezumab, eptinezumab) and oral gepants (atogepant, rimegepant), represent a paradigm shift in episodic migraine prevention. No direct head-to-head trials across the full drug class exist. We conducted a PRISMA-NMA-compliant Bayesian network meta-analysis (NMA) to compare the relative efficacy and tolerability of all […]
Undetected isoniazid resistance leads to rifampin-resistant tuberculosis
Background: Isoniazid resistance is the most common form of drug-resistant tuberculosis (TB) globally. However, WHO-recommended molecular tests available to most TB patients worldwide detect rifampin resistance only, risking under-treatment of isoniazid-resistant, rifampin-susceptible TB (HR-TB) and subsequent emergence of rifampin resistance. Methods: This prospective study (2020-2024) aimed to collect and archive sputum specimens from all adults […]
A RHNO1-ATR/Chk1 positive feedback loop sustains the DNA replication stress response
Elevated DNA replication stress is a common feature of cancer cells, rendering them dependent on ATR/Chk1 signaling, which controls the DNA replication stress checkpoint, for survival. Although the activation of ATR/Chk1 signaling is well-established, how this signal is maintained until replication stress is fully resolved is less understood. Here, we investigated the roles of RAD9 […]
Argonaute-2 slicing of Rtl1 promotes skeletal muscle development and postnatal survival
Argonaute (AGO) proteins associate with small guide RNAs to bind and repress mRNA targets. AGO2 is the primary AGO slicer in mammals, cleaving RNAs that base-pair extensively to its guide RNA. Disrupting the slicing activity of AGO2 in mice causes neonatal lethality, however, the specific cell types and substrates that contribute to this lethality are […]
FBH1 and RAD54L directly interact and cooperate to drive replication fork reversal
Replication fork reversal alleviates DNA replication stress and maintains genome stability. We previously showed that FBH1 and RAD54L cooperate to promote fork reversal in human cells, and that FBH1-dependent fork reversal requires the branch migration activity of RAD54L. However, the molecular basis of this cooperation remained unclear. Here, we identify both a physical and functional […]
FBH1 and RAD54L directly interact and cooperate to drive replication fork reversal
Replication fork reversal alleviates DNA replication stress and maintains genome stability. We previously showed that FBH1 and RAD54L cooperate to promote fork reversal in human cells, and that FBH1-dependent fork reversal requires the branch migration activity of RAD54L. However, the molecular basis of this cooperation remained unclear. Here, we identify both a physical and functional […]
A RHNO1-ATR/Chk1 positive feedback loop sustains the DNA replication stress response
Elevated DNA replication stress is a common feature of cancer cells, rendering them dependent on ATR/Chk1 signaling, which controls the DNA replication stress checkpoint, for survival. Although the activation of ATR/Chk1 signaling is well-established, how this signal is maintained until replication stress is fully resolved is less understood. Here, we investigated the roles of RAD9 […]
Argonaute-2 slicing of Rtl1 promotes skeletal muscle development and postnatal survival
Argonaute (AGO) proteins associate with small guide RNAs to bind and repress mRNA targets. AGO2 is the primary AGO slicer in mammals, cleaving RNAs that base-pair extensively to its guide RNA. Disrupting the slicing activity of AGO2 in mice causes neonatal lethality, however, the specific cell types and substrates that contribute to this lethality are […]
Predicting the median lifespan of ITNs using an area-measure of risk of physical durability: proof of concept of the Risk Index
Background: The lifespan of insecticide-treated nets (ITNs) varies widely across settings, reflecting both intrinsic product characteristics and external factors related to use, care, and environment. While the resistance to damage (RD) score captures intrinsic product durability, there is no standardized metric to quantify contextual risks. This study presents a proof of concept for the Risk […]
Clinical effectiveness of SGLT2 inhibitors in non-diabetic kidney transplanted patients- a real world data analysis.
Background and hypothesis: Sodium-glucose cotransporter-2 inhibitors (SGLT2-inhibitors) slow chronic kidney disease progression, but evidence in non-diabetic kidney transplant recipients is limited. We evaluated associations between SGLT2-inhibitor use and major adverse kidney events (MAKE), major adverse cardiovascular events (MACE), and all-cause mortality. Methods: In this retrospective cohort study using the TriNetX federated research network, adult non-diabetic […]
Determinants of specificity and end-user acceptability of an IP-10-based point-of-care triage test for antiretroviral therapy monitoring in Mozambique
Introduction: Viral load (VL) monitoring is the gold standard for antiretroviral therapy (ART) monitoring. Still, due to limited funds and infrastructure, many people living with HIV (PLHIV) in low- and middle-income countries do not receive timely VL testing. We evaluated the clinical performance and end-user acceptability of a prototype interferon gamma-induced protein 10 (IP-10) point-of-care […]