We describe a method for targeted sequencing of expressed mutations in single cells. The approach exploits the reverse transcriptase and nick translation activities of the Bst DNA polymerase (Full Length) from Bacillus stearothermophilus (BstFL) to perform an RNA-templated gap filling and ligation between flanking probes. Integrated with probe-based single cell RNAseq workflows, it enables simultaneous whole transcriptomic and multiplexed mutation profiling from the same cell, overcoming key limitations of mutation detection in existing scRNA-seq approaches.
Bioethical considerations in deploying mobile mental health apps in LMIC settings: insights from the MITHRA pilot study in rural India
IntroductionIn India, untreated depression among women contributes significantly to morbidity and mortality, underscoring an urgent need for accessible and ethically grounded mental health interventions. Mobile



