Collagen remodeling in the uterine cervix is a vital process in pregnancy that allows for timely fetal delivery, yet its spatio-temporal details are still not fully understood. In this study, we measured collagen reorganization at different stages of murine gestation and at various cervical depths. We used polarization-resolved Second Harmonic Generation microscopy to specifically detect fibrillar collagen and assess its orientation with sub-micrometer resolution. We imaged large cervical areas using automated mosaicking and implemented an analysis pipeline that showed significant region-dependent changes in collagen quantity, porosity, and orientation disorder. Notably, we found that collagen disorganization begins in the lower cervix at gestation day 12 and extends throughout the entire cervix by day 15. Additionally, we demonstrated that the temporal dynamics of disorganization, without spatial sensitivity, can also be tracked using Mueller Matrix imaging, which is a clinically deployable method. These findings should improve understanding and diagnosis of gestation-related issues such as premature birth.
Toward terminological clarity in digital biomarker research
Digital biomarker research has generated thousands of publications demonstrating associations between sensor-derived measures and clinical conditions, yet clinical adoption remains negligible. We identify a foundational