arXiv:2512.20805v1 Announce Type: new
Abstract: The cryopreservation of biological materials is a highly complex process, as it involves numerous factors such as the cooling and thawing procedures, the administration of cryoprotective agents (CPAs), as well as the type and composition of cells. While theoretical work has yielded a better understanding of the processes occurring during cryopreservation, the design of cryopreservation protocols and their parameters is currently predominantly based on heuristic optimization. Here, we propose a mathematical method to optimise the cooling dynamics in slow-cooling, to reduce the risk of injury. We derive our method from first principles and provide computational predictions. Moreover, we assess the predictions with data obtained from the literature, as well as novel experimental results. Overall, we provide a generic computational approach to generate improved slow-cooling profiles for the cryopreservation of cells in suspension.
Just-In-Time Adaptive Interventions for Weight Management Among Adults With Excess Body Weight: Scoping Review
Background: Just-in-time adaptive interventions (JITAIs) use real-time monitoring to deliver personalized support at optimal moments, demonstrating potential for improving lifestyle behaviors in weight management. Objective: