Single-cell DNA methylation sequencing has remained technically specialized due to challenges of interfacing conversion chemistry with droplet microfluidics. We introduce pre-tagmentation in situ conversion (PreTIC), which enables whole-methylome profiling on a commercial droplet platform using off-the-shelf reagents. With PreTIC, we produce over 13,000 single-cell methylomes from fixed frozen cells in two days and resolve cell-specific epigenetic variation in human peripheral blood mononuclear cells.
Measuring and reducing surgical staff stress in a realistic operating room setting using EDA monitoring and smart hearing protection
BackgroundStress is a critical factor in the operating room (OR) and affects both the performance and well-being of surgical staff. Measuring and mitigating this stress